Separation of Some Aromatic Amino Acid Enantiomers with Pressurized Planar Electrochromatography and TLC |
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Authors: | Beata Polak Krzysztof K Wojtanowski Piotr ?l?zak Tadeusz H Dzido |
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Institution: | 1. Department of Physical Chemistry, Medical University of Lublin, Chod?ki 6, 20-093, Lublin, Poland
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Abstract: | Influence of buffer pH, buffer concentration and ratio of methanol to acetonitrile in ternary mobile phase on migration distance of enantiomers of phenylalanine, tyrosine and DOPA in pressurized planar electrochromatography (PPEC) and thin-layer chromatography (TLC) systems is presented and compared. The applied operating variables in PPEC system more strongly influence on separation selectivity of investigated solutes than those in TLC one. Higher separation efficiency of PPEC system relative to TLC one is also demonstrated. |
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