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Determination of Propylene Glycol in Low Volume Plasma and Urine Samples of Neonates by LC with Photodiode Array Detection |
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| Authors: | Aida Kulo Karel Allegaert Jan de Hoon Rene Verbesselt |
| Institution: | 1. Center for Clinical Pharmacology, University Hospitals Leuven, Campus Gasthuisberg, Herestraat 49, 3000, Leuven, Belgium 2. Institute of Pharmacology, Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Sarajevo, ?ekalu?a 90, 71000, Sarajevo, Bosnia and Herzegovina 3. Neonatal Intensive Care Unit, University Hospitals Leuven, Campus Gasthuisberg, Herestraat 49, 3000, Leuven, Belgium |
| Abstract: | In order to enhance the sensitivity and to develop a method suitable for quantification of propylene glycol (PG) in low volume neonate plasma and urine samples, several steps in earlier described high performance liquid chromatographic methods were optimised. Chromatographic separation on a reversed-phase column and ultraviolet detection resulted in cleaner chromatograms without interfering compounds. Linearity of the standard curves was validated in the concentration range 0.25?C50 mg L?1. The lower limit of quantification was 20 times lower than in earlier described methods. Presented method was suitable for quantification of PG concentrations in low volume neonate plasma (15?C46 mg L?1) and urine samples (20?C175 mg L?1) enabling us to document very low renal versus non-renal contribution of PG clearance in neonates. |
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