A Simple Method for Monitoring Iodinated Casein in Feed Premixes by LC?CMS Using Iodothyronines as Markers |
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| Authors: | Zongyi Wang Wenjun Yang Biying Chang Xiangning Chen Liying Zhang Lihua He |
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| Institution: | 1. State Key Laboratory of Animal Nutrition, China Agricultural University, 100193, Beijing, People??s Republic of China
2. Faculty of Food Science, Beijing University of Agricluture, 102206, Beijing, People??s Republic of China
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| Abstract: | A simple and effective liquid chromatography?Cmass spectrometry method was devoleped for mornitoring iodinated casein (IC) in feed premixes. In this method, iodothyronines released from IC upon sample hydrolysis were used as markers. Samples were treated with Na2S and hydrolyzed with Ba(OH)2??H2O followed by cleaned up using a SampliQ SPE cartridge. Chromatographic separation was achieved on a C18 column with isocratic elution using water (0.1% formic acid, v/v) and acetonitrile as the mobile phase. Ion detection was performed using electrospray ionization in positive mode using selected ion monitoring at m/z 606, 652, 732 and 778. IC levels were mornitored by qualitative analysis of the iodothyronines and quantification of thyroxine. Validation data demonstrated that the method was selective and sensitive (??0.5?mg?g?1) with acceptable accuracy (70.2?C128.2% recovery) and precison (RSD 5.5?C15.2%). |
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