AFM imaging of RGD presenting synthetic extracellular matrix using gold nanoparticles |
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Authors: | Hsiong Susan X Cooke Peter H Kong Hyun-Joon Fishman Marshall L Ericsson Maria Mooney David J |
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Affiliation: | Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA. |
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Abstract: | Several high-resolution imaging techniques such as FESEM, TEM and AFM are compared with respect to their application on alginate hydrogels, a widely used polysaccharide biomaterial. A new AFM method applicable to RGD peptides covalently conjugated to alginate hydrogels is described. High-resolution images of RGD adhesion ligand distribution were obtained by labeling biotinylated RGD peptides with streptavidin-labeled gold nanoparticles. This method may broadly provide a useful tool for sECM characterization and design for tissue regeneration strategies. |
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Keywords: | atomic force microscopy (AFM) biomaterials electron microscopy hydrogels TEM |
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