Gd3+对原代培养的小鼠骨髓基质细胞成骨分化和成脂分化的影响

本文采用MTT法、碱性磷酸酶活性测定、矿化功能的测定以及油红O的染色和定量测定等手段研究了Gd3+对原代培养的小鼠骨髓基质细胞成骨分化和成脂分化的影响。研究结果表明,浓度为1×10-10和1×10-8 mol.L-1的Gd3+对小鼠骨髓基质细胞的增殖没有影响,其他测试浓度下的Gd3+则抑制小鼠骨髓基质细胞的增殖。当Gd3+与小鼠骨髓基质细胞作用7 d时,其对小鼠骨髓基质细胞成骨分化的影响与作用浓度有关,当Gd3+与小鼠骨髓基质细胞作用14 d时,在全部测试浓度范围内,抑制小鼠骨髓基质细胞成骨分化。除1×10-8和1×10-5 mol.L-1外,其他测试浓度下的Gd3+促进小鼠骨髓基质细胞的矿化功能。当Gd3+与小鼠骨髓基质细胞作用10 d时,其抑制小鼠骨髓基质细胞的成脂分化,当Gd3+与小鼠骨髓基质细胞作用16 d时,除1×10-9mol.L-1外,其他浓度的Gd3+也抑制小鼠骨髓基质细胞的成脂分化。实验结果提示,Gd3+可能通过促进骨髓基质细胞的成骨分化、抑制其成脂分化途径起到对骨的保护作用。Gd3+对原代培养的小鼠骨髓基质细胞成骨分化和成脂分化的影响与作用浓度和时间有关,而且,它们是影响Gd3+对骨是损伤还是保护作用转变的关键因素。

Effects of Gd3+ on Osteogenic and Adipogenic Differentiation of Mouse Primary Bone Marrow Stromal Cells

A series of experimental assays including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test, alkaline phosphatase (ALP) activity measurement, mineralized function, oil red O stain and measurement were employed to assess the effects of Gd³⁺ on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells (BMSCs). The results indicate that Gd³⁺ has no effect on the proliferation of BMSCs at concentrations of 1×10^-10 and 1×10^-8 mol·L^-1, but inhibits the proliferation at other concentrations. The effect of Gd³⁺ on the osteogenic differentiation depends on concentrations at the 7th day, but Gd³⁺ inhibits the osteogenic differentiation at any concentration at the 14th day. Gd³⁺ can promote the formation of mineralized matrix nodules except at concentrations of 1×10^-8 and 1×10^-5 mol·L^-1. Gd³⁺ can inhibit the adipogenic differentiation at any concentration at the 10th day, but inhibit the adipogenic differentiation except at a concentration of 1×10^-9 mol·L^-1 at the 16th day. These findings suggest Gd³⁺ may have protective effect on bone at appropriate dose by decreasing adipogenic differentiation and promoting osteogenic differentiation of BMSCs. The effects of Gd³⁺ on the osteogenic and adipogenic differentiation of BMSCs depend on the concentration and culture time, moreover, they are pivotal factors for switching the biological effects of Gd³⁺ from damage to protection.