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Selective carboxylation of substituted phenols with engineered Escherichia coli whole-cells
Authors:Chang Peng  Yuxue Liu  Xiaojia Guo  Wujun Liu  Qing Li  Zongbao K Zhao
Institution:1. Division of Biotechnology, Dalian Institute of Chemical Physicals, Chinese Academy of Sciences, Dalian 116023, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. State Key Laboratory of Catalysis, Dalian Institute of Chemical Physicals, CAS, Dalian 116023, China
Abstract:Selective carboxylation of substituted phenols is realized in the presence of bicarbonate under ambient pressure by engineered Escherichia coli whole-cells expressing various hydroxybenzoate decarboxylases, leading to their corresponding ortho-hydroxybenzoates. This process may be further developed as an efficient route to upgrade lignin-derived phenolic compounds as valuable building blocks.
Keywords:Carboxylation  5-Carboxyvanillate decarboxylase  Lignin-derived phenols  Decarboxylase  Whole-cell biocatalyst
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