Liquid-phase microextraction based on carrier mediated transport combined with liquid chromatography-mass spectrometry. New concept for the determination of polar drugs in a single drop of human plasma |
| |
Authors: | Ho Tung Si Reubsaet Jan Leon Egge Anthonsen Hanne Sofie Pedersen-Bjergaard Stig Rasmussen Knut Einar |
| |
Affiliation: | School of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, N-0316 Oslo, Norway. tungsho@farmasi.uio.no |
| |
Abstract: | Recently, we demonstrated for the first time liquid-phase microextraction (LPME) of polar drugs based on carrier mediated transport. In this new extraction technique, selected analytes were extracted as ion-pairs from small volumes of biological samples, through a thin layer of a water immiscible organic solvent immobilised in the pores of a porous hollow fibre (liquid membrane), and into a microl volume of an acidic aqueous acceptor solution placed inside the lumen of the hollow fibre. In the current paper, this new extraction technique was combined with liquid chromatography-mass spectrometry (LC-MS) for the first time. Carrier mediated LPME was evaluated for several new model drugs (0.01
|
| |
Keywords: | Liquid-phase microextraction Carrier mediated transport Drugs Polar |
本文献已被 ScienceDirect PubMed 等数据库收录! |