| 牛血清白蛋白与光谱探针相互作用机理的研究 |
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| 引用本文： | 谭帼馨,崔英德,肖楚民. 牛血清白蛋白与光谱探针相互作用机理的研究[J]. 分析试验室, 2005, 24(10): 26-29 |
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| 作者姓名： | 谭帼馨 崔英德 肖楚民 |
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| 作者单位： | 广东工业大学轻工化工学院,广州,510006;仲恺农业技术学院,广州,510225 |
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| 基金项目： | 国家自然科学基金;广东省教育厅自然科学基金;广东工业大学校科研和教改项目 |
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| 摘 要： | 在酸性条件下研究了考马斯亮蓝光谱探针与牛血清白蛋白结合物的光学性质，探讨了考马斯亮蓝染料和蛋白质的相互作用机理。研究结果表明染料考马斯亮蓝的疏水作用和亲水作用的协调作用以及与生物大分子蛋白质具有相反电荷的静电作用，是光谱探针与生物大分子形成大分子聚集体的必要因素。
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| 关 键 词： | 分光光度法 牛血清白蛋白 光谱探针 考马斯亮蓝 相互作用机理 |
| 文章编号： | 1000-0720（2005）10-0026-04 |
| 收稿时间： | 2004-09-20 |
| 修稿时间： | 2004-09-202004-11-01 |
Study on interaction between bovine serum albumin and spectral probe |
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| TAN Guo-xin, XIAO Chumin,CUI Ying-de. Study on interaction between bovine serum albumin and spectral probe[J]. Chinese Journal of Analysis Laboratory, 2005, 24(10): 26-29 |
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| Authors: | TAN Guo-xin XIAO Chumin CUI Ying-de |
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| Affiliation: | 1 Faculty of Chemical Engineering and Light Industry Guangdong University of Technology, Guangzhou510006; 2 Zhongkai Agrotechnical College, Guangzhou 510025 |
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| Abstract: | The spectral characteristics of Commassie brilliant blue (CBBG) with bovine serum albumin (BSA) were studied in acidic solutions. The mechanism of the interaction between CBBG and BSA was also investigated. The results show that hydrophobic and hydrophilic functions of CBBG and the electrostatic interaction between CBBG and BSA are essential for formation of macromolecular aggregation between the spectral probe and the biomacomolecules. |
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| Keywords: | Spectrophotometry Bovine serum albumin Spectral probe Commassie brilliant blue Interaction |
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