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Design of enzyme reactors as chromatographic columns for racemic resolution of amino acid esters |
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| Authors: | J Kalbé H Höcker H Berndt |
| Institution: | (1) Lehrstuhl für Textilchemie und Makromolekulare Chemie der RWTH Aachen, Worringer Weg 1, D-5100 Aachen, FRG |
| Abstract: | Summary Racemic resolution of aromatic and aliphatic amino acid esters into L-amino acid and D-amino acid ester via LC and HPLC is achieved by using enzyme reactors as chromatographic columns. For this purpose α-chymotrypsin and trypsin are immobilized on Eupergit C, Sepharose 4B and Lichrosorb-Diol. |
| Keywords: | Column liquid chromatography Chiral stationary phases Immobilized enzymes Racemic resolution of amino acid esters |
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