In vitro study of proteins surface activity by tritium probe |
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Authors: | Maria G Chernysheva Gennadii A Badun |
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Institution: | (1) Chemistry Department, Radiochemistry Division, Lomonosov Moscow State University, 1 Leninskie Gory, 119991 Moscow, Russia |
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Abstract: | A new technique for in vitro studies of biomacromolecules interactions, their adsorption at aqueous/organic liquid interfaces
and distribution in the bulk of liquid/liquid systems was developed. The method includes (1) tritium labeling of biomolecules
by tritium thermal activation method and (2) scintillation phase step with organic phase, which can be concerned as a model
of cellular membrane. Two globular proteins lysozyme and human serum albumin tested. We have determined the conditions of
tritium labeling when labeled by-products can be easy separated by means of dialysis and size-exclusion chromatography. Scintillation
phase experiments were conducted for three types of organic liquids. Thus, the influences of the nature of organic phase on
proteins adsorption and its distribution in the bulk of aqueous/organic liquid system were determined. It was found that proteins
possess high surface activity at aqueous/organic liquid interface. Furthermore, values of hydrophobicity of globular proteins
were found by the experiment. |
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