Synthesis and characterization of photoaffinity probe of acetogenin, a strong inhibitor of mitochondrial complex I |
| |
| Authors: | Shuhei YamamotoMasato Abe Sayo NakanishiMasatoshi Murai Hideto Miyoshi |
| |
| Affiliation: | Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan |
| |
| Abstract: | Acetogenins are valuable inhibitor probes to get an insight into the structural and functional properties of mitochondrial NADH-ubiquinone oxidoreductase (complex I). We synthesized a photoreactive acetogenin mimic ([125I]DANA) which retained a strong inhibitory activity. The preliminary photoaffinity labeling with bovine heart submitochondrial particles revealed that [125I]DANA binds to the ND1 subunit among 45 different subunits with high specificity. |
| |
| Keywords: | Acetogenins Photoaffinity labeling Radioiodination Mitochondrial complex I Respiratory enzyme |
| 本文献已被 ScienceDirect 等数据库收录！ |
