High-performance liquid chromatographic method for simultaneous quantification of eight major biologically active ingredients in 'Da-Chai-Hu-Tang' preparation |
| |
Authors: | Li Chia-Ying Chiu Chiung-Hui Huang Ho-Shin Lin Chung-Hua Wu Tian-Shung |
| |
Affiliation: | Department of Chemistry, National Cheng Kung University, Tainan 701, Taiwan. |
| |
Abstract: | A simple, sensitive and specific high-performance liquid chromatographic method has been developed for the first time to simultaneously determine the eight major biologically active ingredients, namely paeoniflorin, naringin, sennoside A, baicalin, baicalein, saikosaponin a, rhein and emodin of the Chinese herbal formula Da-Chai-Hu-Tang. The contents of these marker substances in Da-Chai-Hu-Tang extract could be easily determined within 85 min. The assay was reproducible and accurate with overall intra-day variations and accuracy of less than 2% and more than 97.9%, respectively. Results indicate that the developed HPLC assay can be successfully utilized as a quality control method for simultaneous determination of eight representative substances in Da-Chai-Hu-Tang. |
| |
Keywords: | Da‐Chai‐Hu‐Tang paeoniflorin naringin sennoside A baicalin baicalein saikosaponin a rhein emodin HPLC |
本文献已被 PubMed 等数据库收录! |
|