Protein expression differs between neural progenitor cells from the adult rat brain subventricular zone and olfactory bulb |
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Authors: | Martin H Maurer Jr" target="_blank">Robert E FeldmannJr Heinrich F Bürgers Wolfgang Kuschinsky |
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Institution: | (1) Department of Physiology and Pathophysiology, University of Heidelberg, Im Neuenheimer Feld 326, 69120 Heidelberg, Germany |
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Abstract: | Background Neural progenitor cells can be isolated from various regions of the adult mammalian brain, including the forebrain structures
of the subventricular zone and the olfactory bulb. Currently it is unknown whether functional differences in these progenitor
cell populations can already be found on the molecular level. Therefore, we compared protein expression profiles between progenitor
cells isolated from the subventricular zone and the olfactory bulb using a proteomic approach based on two-dimensional gel
electrophoresis and mass spectrometry. The subventricular zone and the olfactory bulb are connected by the Rostral Migratory
Stream (RMS), in which glial fibrillary acidic protein (GFAP)-positive cells guide neuroblasts. Recent literature suggested
that these GFAP-positive cells possess neurogenic potential themselves. In the current study, we therefore compared the cultured
neurospheres for the fraction of GFAP-positive cells and their morphology of over a prolonged period of time. |
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