Evaluation of ultra-performance liquid chromatography in the bioanalysis of small molecule drug candidates in plasma |
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| Authors: | Goodwin Lee White Stephen A Spooner Neil |
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| Institution: | Worldwide Bioanalysis, Glaxosmithkline Research and Development, Park Road,Ware, Hertfordshire, SG12 0DP, UK. lee.x.goodwin@gsk.com |
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| Abstract: | Ultra-performance liquid chromatography (UPLC) combined with mass spectrometric detection (MS) is used successfully in the bioanalysis of small molecule drug candidates in plasma. UPLC-MS is shown to increase sample throughput by reducing run times over 3-fold, without compromising analytical sensitivity or analyte resolution. The technique is demonstrated to be practical and robust on a commercially available ultra-high pressure system when injecting extracts of plasma and has also shown to be a technique that can be used effectively on a conventional high-performance liquid chromatography system fitted with short columns (
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