Growth kinetics and phenolics production inGlycine Max cell suspension cultures |
| |
Authors: | Shetty Kalidas Korus roger A Crawford Dom L |
| |
Institution: | 1.Department of Bacteriology and Biochemistry, Moscow ;2.Department of Chemical Engineering, University of Idaho, 83843, Moscow, ID ; |
| |
Abstract: | Glycine max was used as a model plant cell suspension culture to establish relationships among growth kinetics, phenolics production,
elicitor action, and peroxidase activity. Timing of elictor addition through monitoring of peroxidase provided an excellent
means of optimizing yields of phenolics and reduced the time span during which phenolics were formed, negating the need for
a secondary production medium. We have also determined that calcium and other cellular effectors like polyamines and organic
osmolytes, when used in conjunction with elicitors, enhance phenolics production. Calcium directly enhanced elicitation, whereas
polyamines and other osmolytes such as glycerol and proline extended cell viability. The study also demonstrated potential
for enhancing secondary metabolite production by a combination of elicitation, cell viability stabilizers, and by addition
of nutrients at the time of elicitation. |
| |
Keywords: | Index Entries" target="_blank">Index Entries Phenolics glycine max plant cell culture L-pheny-lalanine ammonia-lyase" target="_blank">L-pheny-lalanine ammonia-lyase elicitors calcium polyamines osmolytes |
本文献已被 SpringerLink 等数据库收录! |
|