Recombinant cellulose crosslinking protein: a novel paper-modification biomaterial |
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| Authors: | Ilan levy Amos Nussinovitch Etai Shpigel Oded Shoseyov |
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| Institution: | (1) The Kennedy Leigh Center for Horticultural Research and The Otto Warburg Center for Agricultural Biotechnology, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel;(2) Institute of Biochemistry, Food Science and Human Nutrition, the Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel;(3) The Kennedy Leigh Center for Horticultural Research and The Otto Warburg Center for Agricultural Biotechnology, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel |
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| Abstract: | Cellulose-binding domains have been isolated from various cellulases, and proteins, which lack hydrolytic activity. The hypothesis that a cellulose-binding domain can be used to alter surface and mechanical properties of paper was tested. Two cellulose-binding domains from Clostriium cellulovorans were fused to form a cellulose crosslinking protein (CCP). The recombinant bifunctional cellulose-binding protein was expressed in E. coli, appliedby immersion onto Whatman cellulose filter paper, and its mechanical properties were tested. The purified protein improved the treated paper's mechanical properties (tensile strength, brittleness, Young's modulus and energy to break). In addition, cellulose crosslinking protein treatment was shown to transform filter paper into a more water-repellent paper. The binding of cellulose-binding domains to cellulose under a wide range of envi-ronmentalconditions, without the need for chemical reactions, and its biodegradability make them attractive moieties for the design of a new class of paper-modification materials.p> |
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| Keywords: | Cellulose crosslinking protein Cellulose fibers Cellulose-binding domain Mechanical properties Paper |
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