Characterization and quantification of 10‐hydroxycamptothecine in Camptotheca acuminate and its medicinal preparation by liquid chromatography–ion trap mass spectrometry |
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Authors: | Qinhua Chen Guangmin Pan Lin Xiong Hongsheng He Hangdong Yang |
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Affiliation: | Institute of Pharmaceutical Analysis and Drug Screening, Affiliated Dongfeng Hospital, Hubei University of Medicine, , Hubei, 442008 China |
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Abstract: | A rapid and sensitive method for the identification and quantification of 10‐hydroxycamptothecine (HCPT) in Camptotheca acuminata Decne is described. The HCPT standard solution was directly infused into the ion trap mass spectrometers (IT/MS) for collecting the MSn spectra. The electrospray ionization (ESI) mass spectral fragmentation pathway of HCPT was proposed and the ESI‐MSn fragmentation behavior of HCPT was deduced in detail. The major fragment ions of HCPT were confirmed by MSn in both negative ion and positive ion mode. The possible main cleavage pathway of fragment ions was studied. Quantification of HCPT was assigned in negative‐ion mode at a product ion at m/z 363 → 319 by LC‐MS. The LC‐MS method was validated for linearity, sensitivity, accuracy and precision, and then used to determine the content of the HCPT. Lastly, the LC‐MS method was successfully applied to determine HCPT in real samples of Camptotheca acuminate Decne and its medicinal preparation in the first time. Copyright © 2013 John Wiley & Sons, Ltd. |
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Keywords: | liquid chromatography– ion trap mass spectrometry 10‐hydroxycamptothecine fragmentation pathways Camptotheca acuminate Decne |
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