Chiral analytical method development of liquiritigenin with application to a pharmacokinetic study |
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Authors: | Casey L Sayre Mandi Hopkins Jody K Takemoto Neal M Davies |
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Institution: | 1. Faculty of Pharmacy, University of Manitoba, , Winnipeg, Manitoba, Canada, R3E 0T5;2. College of Pharmacy, Washington State University, , Pullman, WA, 99164‐6534 USA;3. John A. Burns School of Medicine, Department of Cell and Molecular Biology, University of Hawaii, , Honolulu, HI, 96813 USA |
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Abstract: | Pharmacometric characterization studies of liquiritigenin have historically overlooked its chiral nature. To achieve complete characterization, an analytical method enabling the detection and quantification of the individual enantiomers of racemic (±) liquiritigenin is necessary. Resolution of the enantiomers of liquiritigenin was achieved using a simple high‐performance liquid chromatographic method. A Chiralpak® ADRH column was employed to perform baseline separation with UV detection at 210 nm.The standard curves were linear ranging from 0.5 to 100 µg/mL for each enantiomer. Limit of quantification was 0.5 µg/mL. The assay was applied successfully to stereoselective serum disposition of liquiritigenin enantiomers in rats. Liquiritigenin enantiomers were detected in serum as both aglycones and glucuronidated conjugates. Both unconjugated enantiomers had a serum half‐life of ~15 min in rats. The volume of distribution (Vd) for S‐ and R‐liquiritigenin was 1.49 and 2.21 L/kg, respectively. Total clearance (Cltotal) was 5.12 L/h/kg for S‐liquiritigenin and 4.79 L/h/kg for R‐liquiritigenin, and area under the curve (AUC0‐inf) was 3.95 µg h/mL for S‐liquiritigenin and 4.23 µg h/mL for R‐liquiritigenin. The large volume of distribution coupled with the short serum half‐life suggests extensive distribution of liquiritigenin into tissues. Copyright © 2012 John Wiley & Sons, Ltd. |
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Keywords: | reversed‐phase HPLC liquiritigenin stereospecific pharmacokinetics flavonoid |
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