Ultra‐performance liquid chromatography‐tandem mass spectrometry method for the determination of febuxostat in dog plasma and its application to a pharmacokinetic study |
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Authors: | Tianhong Zhang Yuanpeng Sun Peng Zhang Jingmei Gao Shanshan Wang Zhonggui He |
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Affiliation: | 1. School of Pharmacy, Shenyang Pharmaceutical University, , Shenyang, 110016 China;2. Yangtze River Pharmaceutical Group, , Taizhou, Jiangsu, 225321 China |
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Abstract: | A rapid, sensitive and selective ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed and validated for the determination of febuxostat in dog plasma. Using paclitaxel as an internal standard (IS), a simple liquid–liquid extraction method with ethyl acetate was adopted for plasma sample pretreatment. Separation was carried out on an Acquity UPLC BEH C18 column with a mobile phase consisting of acetonitrile and water (containing 0.2% formic acid). The assay was linear in the concentration ranged from 5 to 5000 ng/mL with a lower limit of quantification of 5 ng/mL for febuxostat. The single run analysis was as short as 2.0 min. Finally, the developed method was successfully applied to the pharmacokinetic study of febuxostat tablets following oral administration at a single dose of 40 mg in beagle dogs. Copyright © 2012 John Wiley & Sons, Ltd. |
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Keywords: | UPLC– MS/MS febuxoatat liquid– liquid extraction pharmacokinetic study |
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