The biotransformation of oleuropein in rats |
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| Authors: | Peiyan Lin Weidong Qian Xiaoying Wang Li Cao Shaofen Li Tianxiu Qian |
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| Affiliation: | 1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, , Beijing, 100193 People's Republic of China;2. Department of Neurology, The First Affiliated Hospital of Bengbu Medical College, , Bengbu, 233004 People's Republic of China;3. Beijing Chao‐Yang Hospital, Capital Medical University, , Beijing, 100020 People's Republic of China |
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| Abstract: | A highly sensitive and specific LC‐MS/MS method was developed to investigate the in vivo bio‐transformation of oleuropein in rat. Rat feces and urine samples collected after oral administration were determined by liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the negative‐ion mode. The assay procedure involves a simple liquid–liquid extraction of parent oleuropein and the metabolite from rat feces and urine with ethyl acetate. Chromatographic separation was operated with 0.1% formic acid aqueous and methanol in gradient program at a flow rate of 0.50 mL/min on an RP‐C18 column with a total run time of 31 min. This method was successfully applied to simultaneous determination of oleuropein and its metabolites in rat feces and urine. De‐glucosylation, hydrolysis, oxygenation and methylation were found to comprise the major metabolic pathway of oleuropein in rat gastrointestinal tract and three metabolites were absorbed into the blood circulatory system within 24 h after oral administration. Copyright © 2013 John Wiley & Sons, Ltd. |
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| Keywords: | oleuropein in vivo metabolism feces urine LC‐MS/MS |
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