Independent exponential feeding of glycerol and methanol for fed-batch culture of recombinant Hansenula polymorpha DL-1 |
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Authors: | H. Moon S. W. Kim J. Lee S. K. Rhee E. S. Choi H. A. Kang I. H. Kim S. I. Hong |
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Affiliation: | (1) Graduate School of Biotechnology, Korea University, 136-701 Soul, Korea;(2) Department of Chemical and Biochemical Engineering, Korea University, 136-701 Seoul, Korea;(3) Korea Research Institute of Biosience and Biotechnology, 305-600 Taejon, Korea |
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Abstract: | As a novel feeding strategy for aptomizing human epidermal growth factor (hEGF) production with a recombinant Hansenula polymorpha DL-1 using the methanol oxidase (MOX) promoter in H. polymorpha DL-1, independent exponential feeding of two substrates was used. A simple kinetic model considering the cell growth on two substrates was established and used to calculate the respective feeding rates of glycerol and methanol. In the fedbatch culture with methanol-only feeding, the optimal set point of specific growth rate on methanol was found to be 0.10 h−1. When the fed-batch cultures were conducted by the independent feeding of glycerol and methanol, the actual specific growth rate on glycerol and methanol was slightly lower than the set point of specific growth rate. By the uncoupled feeding of glycerol and methanol the volumetric productivity of hEGF increased from 6.4 to 8.0 mg/(L·h), compared with methanol-only feeding. |
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Keywords: | Hansenula polymorpha human epidermal growth factor fed-batch culture exponential feeding mixed feed |
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