Biodegradation of n-hexadecane by bacterial strains B1 and B2 isolated from petroleum-contaminated soil |
| |
| Authors: | Liu Tao Wang FengHua Guo LanPing Li XiaoLiang Yang XiaoJin Lin Ai Jun |
| |
| Institution: | Key Laboratory of Pollution Control and Resource Reuse(Tongji University),Shanghai 200092,China 2 Experimental Teaching Center of Environmental Science and Engineering,Tongji University,Shanghai 200092,China 3 Department of Environmental Science and Engineering,Beijing University of Chemical Technology,Beijing 100029,China 4 Department of Soil Environmental Sciences,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China 5 Institute of Chinese Material Medical,China Academy of Chinese Medical Sciences,Beijing 100700,China 6 Chinese Academy for Environmental Planning,Beijing 100012,China |
| |
| Abstract: | Two hydrocarbon-biodegrading bacterial strains,B1 and B2,were isolated from petroleum-contaminated soil collected from Tianjin,China.The strains were identified as Pseudomonas aeruginosa(B1) and Acinetobacter junii(B2).The degradation rate of n-hexadecane by B1 and B2 reached 96% and 78% respectively after 7 days,though the strains employed different mechanisms of degradation.The results showed that B2 was not able to use glucose as carbon source.B1 could produce glycolipid surfactants using glucose as the carbon source,according to the results of blue agar plate analysis and thin layer chromatography(TLC),and the bacterial culture of B1 had a high oil discharge and emulsification activity.Both B1 and B2 could produce biosurfactants with hexadecane as the sole carbon source,but their modes of action were different.The carbon source was found to affect the cell surface hydrophobicity.Cell surface hydrophobicity was poor with glucose as the carbon source,but enhanced when hexadecane was used as the carbon source. |
| |
| Keywords: | hexadecane biosurfactant hydrophobicity emulsification bacteria |
| 本文献已被 CNKI SpringerLink 等数据库收录！ |
|
