Preparative isolation and purification of three flavonoids from the Chinese medicinal plant Epimedium koreamum Nakai by high-speed counter-current chromatography |
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Authors: | Liu Renmin Li Aifeng Sun Ailing Cui Jichun Kong Lingyi |
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Institution: | Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China. |
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Abstract: | A preparative high-speed counter-current chromatography (HSCCC) method for isolation and purification of flavonoids from the Chinese medicinal plant Epimedium koreamum Nakai was successfully established by using chloroform-methanol-water (4:3.5:2, v/v) as the two-phase solvent system. The method yielded 11.4 mg of epimedokoreanoside I, 46.5 mg of icariin and 17.7 mg of icariside II from 200 mg of the crude sample in one-step separation with the purity of 98.2%, 99.7% and 98.5%, respectively, as determined by high-performance liquid chromatography (HPLC). The structures of the flavonoids were identified by 1H NMR and 13C NMR. |
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Keywords: | Counter-current chromatography Epimedokoreanoside I Icariin Icariside II |
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