| 基于磺酸酯的荧光探针用于活细胞内过氧化氢的成像检测 |
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| 引用本文： | 徐克花,刘芬,汪会霞,王姗姗,王璐璐,唐波. 基于磺酸酯的荧光探针用于活细胞内过氧化氢的成像检测[J]. 中国科学B辑, 2009, 39(6): 473-480 |
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| 作者姓名： | 徐克花 刘芬 汪会霞 王姗姗 王璐璐 唐波 |
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| 作者单位： | 山东师范大学化学化工与材料科学学院;分子与纳米探针教育部重点实验室;农药、医药中间体清洁生产教育部工程研究中心; |
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| 基金项目： | 国家重点基础研究发展计划(批准号:2007CB936000);;国家杰出青年基金(批准号:20725518);;国家自然科学基金重大研究计划(批准号:90713019);;国家自然科学基金(批准号:20875057);;山东省自然科学基金(批准号:Y2007B02);;山东省科技发展计划(批准号:2008GG30003012)项目资助 |
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| 摘 要： | 基于过氧化氢（H2O2）特异性催化水解磺酸酯，设计合成了新型绿色荧光探针：荧光素二磺酸酯（FS—1）和二氯荧光素二磺酸酯（FS-2）两种螺环内酯型化合物，用于活细胞内过氧化氢的检测．探针结构由元素分析、IR、^1H NMR及^13C NMR表征．实验表明：探针FS-1和FS-2在模拟生物体系中检测过氧化氢具有良好的选择性和灵敏度，且线性范围较宽．细胞成像显示：探针FS-1和FS-2用于PMA刺激或外加不同浓度H2O2孵育的小鼠腹膜巨噬细胞均呈现明亮的绿色荧光，且能对细胞内H2O2微摩尔级浓度变化产生响应，证明两探针均具有良好的膜渗透性、高的选择性及良好的灵敏度．该方法的建立对研究生物体内H2O2的产生，H2O2导致的各种疾病机制及H2O2介导的信号转导途径具有重要的理论及实际意义．
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| 关 键 词： | 荧光素二磺酸酯 荧光探针 细胞成像 过氧化氢 |
| 收稿时间： | 2008-12-24 |
| 修稿时间： | 2009-02-12 |
Sulfonate-based fluorescent probes for imaging hydrogen peroxide in living cells |
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| XU KeHua,LIU Fen,WANG HuiXia,WANG ShanShan,WANG LuLu , TANG Bo College of Chemistry,Chemical Engineering , Materials Science,Engineering Research Center of Pesticide , Medicine Intermediate Clean Production,Ministry of Education,Key Laboratory of Molecular , Nano Probes,Sh,ong Normal University,Jinan ,China. Sulfonate-based fluorescent probes for imaging hydrogen peroxide in living cells[J]. Science in China(Series B), 2009, 39(6): 473-480 |
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| Authors: | XU KeHua LIU Fen WANG HuiXia WANG ShanShan WANG LuLu & TANG Bo College of Chemistry Chemical Engineering Materials Science Engineering Research Center of Pesticide Medicine Intermediate Clean Production Ministry of Education Key Laboratory of Molecular Nano Probes Sh ong Normal University Jinan China |
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| Affiliation: | XU KeHua,LIU Fen,WANG HuiXia,WANG ShanShan,WANG LuLu & TANG Bo College of Chemistry,Chemical Engineering , Materials Science,Engineering Research Center of Pesticide , Medicine Intermediate Clean Production,Ministry of Education,Key Laboratory of Molecular , Nano Probes,Sh,ong Normal University,Jinan 250014,China |
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| Abstract: | Based on a mechanism of H2O2-mediated hydrolysis of sulfonates,two fluorescein disulfonates compounds (FS-1 and FS-2) are designed and synthesized as highly selective and sensitive fluorescent probes for imaging H2O2 in living cells,and the probes were characterized with elemental analysis,IR,1H NMR and 13C NMR. Upon reaction with H2O2,the probes exhibit strong fluorescence responses and high selectivity for H2O2 over other reactive oxygen species and some biological compounds. Furthermore,the sulfonate-bas... |
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| Keywords: | fluorescein disulfonates fluorescent probe cell imaging hydrogen peroxide |
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