Development of a non-aqueous capillary electrophoresis method with UV-visible and fluorescence detection for phenolics compounds in olive oil |
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Authors: | Godoy-Caballero M P Acedo-Valenzuela M I Durán-Merás I Galeano-Díaz T |
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Institution: | (1) Analytical Chemistry Department, University of Extremadura, 06006 Badajoz, Spain; |
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Abstract: | Response surface methodology has been applied to the optimization of a simple and rapid non-aqueous capillary electrophoresis
method for the separation and determination of several phenolic compounds belonging to the different families present in olive
oil. A Box–Behnken design was employed and a total of 27 experiments were performed using olive oil samples spiked with the
phenols and injected directly in the capillary after dilution 1:1 with 1-propanol. Finally, the background electrolyte (BGE)
was constituted of 25 mM boric acid and 18 mM KOH in a mixture of 74:26 (v/v) 1-propanol/methanol. The hydrophobicity of the BGE allows its miscibility with the olive oil and, as a consequence, the
possibility of characterizing and determining these kinds of compounds in this sample without any pretreatment. A hydrodynamic
injection (6 s, −30 mbar) was applied and the separation was carried out using 35 °C and +20 kV of separation temperature
and voltage, respectively. A capillary with two detection windows for serial online UV and fluorescence detection was satisfactorily
employed. The validation of the method was carried out by setting the calibration curves, and the figures of merit were finally
obtained. A lineal relationship between the corrected peak area and concentration and limits of detection in the order of
micrograms per milliliter were found. |
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