High-performance liquid chromatographic determination of cyanide in human red blood cells by pre-column fluorescence derivatization. |
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| Authors: | A Sano N Takimoto S Takitani |
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| Affiliation: | Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan. |
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| Abstract: | A method for the determination of cyanide in human red cells has been developed. Cyanide was extracted from red cells by adding water and methanol, and then derivatized with 2,3-naphthalene-dialdehyde and taurine to give a fluorescent product, which was determined by reversed-phase high-performance liquid chromatography with fluorescence detection. The recovery of cyanide from red cells was ca. 83%, and the limit of detection was 100 pmol/ml. The mean concentrations of red cell cyanide from ten smokers and from ten non-smokers were 705 and 466 pmol/ml, respectively. The method was also applicable to whole blood. |
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