| Abstract: | A method has been developed for the quantitative determination of triterpene glycosides — stichoposides — in the tissues of the Far Eastern holothurianStichopus japonicus Selenka. The quantitative determination of the combined triterpene glycosides was based on the isolation of a glycosidic fraction from an ethanolic extract on a column of Polikhrom-1 with subsequent spectrophotometry at 268 nm of the product formed as the result of the reaction of the glycosides with 1 N NaOH. The quantitative determination of the individual glycosides is based on the densitometry of slides or thin-layer chromatography in silica gel with the aid of a Lyuman-IUF-1 luminescence microscope.Pacific Ocean Institute of Bioorganic Chemistry, Far Eastern Scientific Center, Academy of Sciences of the USSR, Vladivostok. Pacific Ocean Institute of Oceanography and Fisheries. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 56–59, January–February, 1984. |
