Simultaneous detection and deconvolution of congested NMR spectra containing three isotopically labeled species |
| |
Authors: | Masterson Larry R Tonelli Marco Markley John L Veglia Gianluigi |
| |
Institution: | Department of Chemistry and Biochemistry, University of Minnesota, Minneapolis, Minnesota 55455-0431, USA. |
| |
Abstract: | We present a procedure for isolating subspectra corresponding to individual protein or peptide components in a ternary mixture or complex. Each of the three-component species is labeled differently: species A uniformly with 15N, species B uniformly with 15N and 13C, and species C uniformly with 15N but selectively with 13C' or 13Calpha. By using the dual carbon label selective HSQC (DCLS-HSQC) pulse sequence and exploiting differences in 1J 15N-13C coupling patterns to filter selected 15N resonances from detection during a constant time period, a subspectrum from each species can be generated from three spectra acquired from a single sample. Many important biological pathways involve dynamic interactions among members of multicomponent protein assemblies, and this approach offers a powerful way to monitor such processes. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|