Nonaqueous capillary electrophoresis for the analysis of selected tropane alkaloids in a plant extract |
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| Authors: | S Cherkaoui L Mateus P Christen J -L Veuthey |
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| Institution: | (1) Laboratory of Pharmaceutical Analytical Chemistry, University of Geneva, 1211 Geneva 4, Switzerland |
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| Abstract: | Summary The potential of nonaqueous capillary electrophoresis has been investigated for the separation of structurally similar tropane
alkaloids. The effects of the organic solvent and of electrolyte composition on separation selectivity, migration times, and
efficiency are described. The addition of trifluoroacetic acid to the separation buffer was found beneficial for manipulation
of the order of migration of the two positional isomers littorine and hyoscyamine. Replicate injections under nonaqueous conditions
gave migration time and peak area data of excellent precision. The application of the optimized conditions to the analysis
of hyoscyamine and scopolamine in genetically transformed root cultures ofDatura candida x D. aurea is presented. |
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| Keywords: | Capillary electrophoresis Micellar electrokinetic capillary chromatography Nonaqueous media Tropane alkaloids Hyoscyamine and scopolamine |
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