酿酒酵母产抱最佳条件的研究

本文通过对耐高温酿酒酵母((S. cerevisiae ) HU - TY -1产抱条件的研究.在菌龄, pH值、培养基组成，Zn2+浓度及微量元素几个方面进行了实验。结果表明，HU-TY-1先在含2写葡萄糖,25}g/ml Zn'+的预产抱培养基上培养，于稳定期前期转入含125}g/ml Zn`+ ,1 j0KAc,0.2500酵母抽提物，0. 1写葡萄糖，0. 1写KH}PO‘的产袍培养基上，可获得高的产抱率(45. 92写).另外、在产抱培养基中加入丁二酸等不被细胞代谢的物质作为纷冲剂，加入生物素，肌醇等维生素可大大提高菌株HU-TY--1产3---4抱(3-4抱即一个子囊中有3个或4个抱子)的能力.

The Studies on Sporulation of S. Gerevisiae

Various affecting factors such as culture time,pH value,component of media,Zn concentration and trace elements on the sporulation of thermotolerant-yeast(Scerevisiae)HU-TY-1 were investigated. The result showed that cultivation in defined presporulation-medium containing 2% glucose and 25 g/mg Zn2+followed by sporulation in a medium containing 125g/mg/Zn2+,1% KAc,0.25% yeast extract,0.1 glucose and 0.1% KH2PO4 reached a maxmum sporulation rate of 45.92%. In addition,Bio-tine,Inositol and buffered sporulation medium consisted of an 0.2M solution;of Succinic acid(SUC) resulted in a further increase in yield of threerand four-spored asci.