| 通过HSQC和1H,13C-COSY叠合增强二维谱分辨率的方法 |
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| 引用本文： | 朱传钧,崔育新,姜标.通过HSQC和1H,13C-COSY叠合增强二维谱分辨率的方法[J].波谱学杂志,2013,30(1):86-92. |
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| 作者姓名： | 朱传钧 崔育新 姜标 |
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| 作者单位： | 中国科学院上海高等研究院,上海201210;上海精细化工火炬创新创业园分析中心,上海201512;天然药物及仿生药物国家重点实验室(北京大学),北京,100191;中国科学院上海高等研究院,上海201210;中国科学院上海有机化学研究所,上海200032 |
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| 基金项目： | 国家自然科学基金(重点项目)资助项目(20832007);国家科技重大专项(重大新药创制)资助项目(2009ZX09501-016,2009ZX09501-007) |
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| 摘 要： | 通常二维谱F1维分辨率较差，F2维分辨率较好. 即HSQC实验13C维度分辨率较差，1H维度分辨率较好. 而1H, 13C-COSY实验13C维度分辨率较好，1H维度分辨率较差. 提出一种通过HSQC和1H, 13C-COSY叠合，取较弱的值储存并显示的方法增强分辨率，采用Bruker Topspin软件AU程序实现. 该方法在不明显增加采样时间的情况下，可显著增强分辨率，信噪比也没有降低. 同时抑制了F1噪声. 处理后，谱图美观清晰.
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| 关 键 词： | 核磁共振(NMR) 分辨率 AU程序 叠合 HSQC 1H 13C-COSY |
A Method for Improving the Resolution of Two-Dimensional NMR Spectrum by Overlapping HSQC and 1H,13C-COSY |
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| ZHU Chuan-jun,CUI Yu-xin,JIANG Biao.A Method for Improving the Resolution of Two-Dimensional NMR Spectrum by Overlapping HSQC and 1H,13C-COSY[J].Chinese Journal of Magnetic Resonance,2013,30(1):86-92. |
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| Authors: | ZHU Chuan-jun CUI Yu-xin JIANG Biao |
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| Institution: | 1. Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China; ;
2. Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China; ;
3. Shanghai Torch Innovation Park of Fine Chemical Industry, Analysis Center, Shanghai 201512, China; ;
4. State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100191, China |
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| Abstract: | In traditional two-dimensional (2D) NMR experiments, spectral resolution in the F1 dimension is usually higher than that in the F2 dimension. Therefore, in the HSQC experiments, the spectral resolution in the 13C dimension is lower than that in the 1H dimension. This is the opposite in the 1H, 13C-COSY experiments. In this work, a method for improving the resolution of 2D NMR spectrum was presented, in which HSQC and 1H, 13C-COSY spectra were overlapped, and then the weaker points were selected, saved and displayed. The method was implemented as an AU program within the Bruker Topspin framework. It was demonstrated experimentally that the method could improve spectral resolution, at no cost of signal-to-noise ratio. The method can also be used to suppress F1 noise. |
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| Keywords: | NMR resolution AU program overlap HSQC 1H 13C-COSY |
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