| 橙皮苷及其4种金属配合物与牛血清白蛋白结合作用的研究 |
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| 引用本文： | 陈建秋,梁佳然,钟文英,季荣. 橙皮苷及其4种金属配合物与牛血清白蛋白结合作用的研究[J]. 分析试验室, 2010, 29(1) |
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| 作者姓名： | 陈建秋 梁佳然 钟文英 季荣 |
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| 作者单位： | 中国药科大学分析化学教研室,南京,210009;南京大学环境学院,南京,210093;中国药科大学分析化学教研室,南京,210009;南京大学环境学院,南京,210093 |
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| 基金项目： | 国家自然科学基金(20777033)项目资助 |
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| 摘 要： | 合成了4种橙皮苷的金属配合物,采用紫外-可见、荧光光谱法研究了橙皮苷4种金属配合物与牛血清白蛋白(BSA)的相互作用。由Stern-Volmer方程和双倒数曲线Lineweaver-Burk方程获得了反应的动态猝灭常数、静态猝灭常数,从而判断出橙皮苷-锌与BSA作用机理为静态猝灭,其它配合物与BSA的作用为动态猝灭。并用热力学方法对结合反应的作用力类型做出了推断,橙皮苷-锌与BSA之间的作用力为静电作用。并得出了橙皮苷金属配合物与血清白蛋白作用的结合常数、结合位点数。同时采用同步荧光光谱法研究了橙皮苷金属配合物对BSA构象的影响。
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| 关 键 词： | 橙皮苷金属配合物 牛血清白蛋白 荧光光谱 热力学参数 |
Study on the interaction of hesperidin and four metal-hesperidin coordination compounds with bovine serum albumin |
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| CHEN Jian-qiu,LIANG Jia-ran,ZHONG Wen-ying,JI Rong. Study on the interaction of hesperidin and four metal-hesperidin coordination compounds with bovine serum albumin[J]. Chinese Journal of Analysis Laboratory, 2010, 29(1) |
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| Authors: | CHEN Jian-qiu LIANG Jia-ran ZHONG Wen-ying JI Rong |
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| Affiliation: | 1.Department of Analytical Chemistry;China Phasmaceutical University;Nanjing 210009;2.School of Environment;Najjing University;Nanjing 210093 |
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| Abstract: | Four metal-hesperidin coordination compounds were synthesized,and the binding of four metal-hesperidin coordination compounds to bovine serum albumin(BSA) was investigated by UV-Vis absorption spectrometry and fluorescence spectrometry.According to Stern-Volmer equation and Lineweaver-Burk equation,the quenching constant was obtained.It is confirmed that the combination reaction between hesperidin-Zn and BSA is a static quenching process,and the quenching mechanism between others and BSA is dynamic quenchin... |
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| Keywords: | Metal-hesperidin coordination compounds Bovine serum albumin Fluorescence spectrometry Thermodynamic parameter |
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