MECHANISTIC STUDIES OF THE PHOTOREACTION OF FLAVIN WITH N, N-DIMETHYLPROPARGYLAMINE |
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| Authors: | JÜ RGEN FRANKE,ULRICH OTT,HORST E. A. KRAMER &dagger ,RAINER H. TRABER |
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| Affiliation: | Institut für Physikalische Chemie, Universität Stuttgart, Pfaffenwaldring 55, D-7000 Stuttgart 80, W, Germany;Ciba-Geigy AG, Dyestuffs and Chemicals Division, K-420.205, CH-4002 Basel, Switzerland |
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| Abstract: | Abstract Both photoexcited free flavin and protein-bound flavin react with N, N-dimethylpropargyla-mine (DMPA) to yield dihydroflavin-adducts which are not reoxidizable by oxygen. The mechanism of the photoreaction was reinvestigated by flash photolysis and continuous illumination experiments. We suggest that a mechanism occurs in which a flavosemiquinone and a DMPA radical result in the first photoinduced reaction. The flavin-DMPA adducts are then formed by combinations of the two radicals. |
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