A rapid HPLC method for the determination of carboxylic acids in human urine using a monolithic column |
| |
Authors: | Email author" target="_blank">I??perlingováEmail author L?Dabrowská V?Stránsky M?Tichy |
| |
Institution: | (1) National Institute of Public Health, robárova 48, CS–100 42, 10 Prague, Czech Republic |
| |
Abstract: | A rapid HPLC method for the determination of carboxylic acids in urine samples using a Chromolith Performance RP/18e 100/4.6 with Chromolith Guard Cartridge RP/18e 10/4.6 (Merck KgaA, Darmstadt, Germany) was developed. The method facilitates the simultaneous determination of aromatic hydrocarbon metabolites mandelic acid (MA) and phenylglyoxylic acid (PGA) from styrene and ethylbenzene, hippuric acid (HA) from toluene and 2-, 3-, 4-methylhippuric acids (MHA) from xylene. 3-Hydroxybenzoic acid (3-HBA) was used as internal standard. A chromatographic run is completed within less than 5 min for styrene, ethylbenzene and toluene metabolites, and within 10 min for xylene metabolites. The detection limits are 9 mg L–1 urine for MA, 1.25 mg L–1 urine for PGA, 4.9 mg L–1 urine for HA, 22 mg L–1 urine for 2-MHA, and 18.5 mg L–1 urine for 3-MHA.No significant differences of the MA, PGA and HA concentrations in human urine samples obtained by HPLC chromatography on LiChrosorb RP 18 and on Chromolith RP/18e columns were found. The results were evaluated by using ANOVA.Abbreviations MA
mandelic acid
- PGA
phenylglyoxylic acid
- HA
hippuric acid
- MHA
methylhippuric acid
- 3-HBA
3-hydroxybenzoic acid
- ANOVA
analyses of variance
- GC
gas chromatography
- HPLC
high-performance liquid chromatography |
| |
Keywords: | Carboxylic acids Monolithic column Human urine |
本文献已被 PubMed SpringerLink 等数据库收录! |
|