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Influence of microwave irradiation on DNA hybridization and polymerase reactions
Affiliation:1. NeuroVita Clinic of Interventional Neurology and Rehabilitation Therapy, Moscow, Russia;2. Institute of Image Processing Systems RAS, Samara, Russia;3. ELBRUS Corporation LLC, Moscow, Russia;4. JSC NPP Istok Named After Shokin, Fryazino, Moscow, Russia;5. International Experimental Central Nervous System Injury & Repair (IECNSIR), Department of Surgical Sciences, Anesthesiology & Intensive Care Medicine, Uppsala University Hospital, Uppsala University, Uppsala, Sweden
Abstract:We report herein that exposure of DNA to microwave irradiation at constant temperature leads to faster strand exchange, as compared with same experiments carried out in a water bath at the same temperature. Furthermore, polymerase chain reactions carried out under microwave irradiation were faster than those in a water bath at the same temperature as well. While the causes of these differences are unclear at this time, this research suggests that microwave irradiation can lead to subtle changes in DNA structural dynamics and functions.
Keywords:Microwave irradiation  DNA hybridization  FRET  Polymerase chain reaction
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