TICT based fluorescent probe with excellent photostability for real-time and long-term imaging of lipid droplets |
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| Affiliation: | 1. School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China;2. National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, China;3. Key Laboratory of Biomedical Engineering of Guangdong Province, South China University of Technology, Guangzhou 51006, China;4. State Key Laboratory of Luminescent Materials and Devices, Institute of Polymer Optoelectronic Materials and Devices, South China University of Technology, Guangzhou 510640, China;1. Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Department of Chemistry, Hubei Key Lab on Organic and Polymeric Optoelectronic Materials, Wuhan University, Wuhan, 430072, PR China;2. National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, PR China;1. Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu, Sichuan, 610064, China;2. Analytical & Testing Center, Sichuan University, Chengdu, 610064, China;1. State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, PR China;2. School of Life Sciences, Lanzhou University, Lanzhou, 730000, PR China;3. College of Basic Medical Sciences, Lanzhou University, Lanzhou, 730000, PR China;4. School of Life Sciences, Kyungpook National University, Daegu, 41566, Republic of Korea;1. State Key Laboratory of Luminescent Materials and Devices, Center for Aggregation-Induced Emission, South China University of Technology, Guangzhou, 510640, China;2. Engineering Research Center of Nano-Geomaterials of Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, 388 Lumo Road, Wuhan, 430074, China;3. Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China |
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| Abstract: | Lipid droplets (LDs) are dynamic organelles and involve in various physiological processes by regulation of the storage and metabolism of lipid molecules. The real-time and long-term imaging of LDs’ distribution and movement is critical for investigation of their biological functions. However, current LDs-targeted fluorescent probes suffer from low photostability and high background noise. To tackle these challenges, we herein reported that the red-emissive fluorescent probe DCQTB with twisted intramolecular charge transfer (TICT) characteristics can be used for wash-free imaging of LDs with advantages of fast cell penetration ability, high specificity, excellent photostability, and low phototoxicity. This LDs-specific fluorescent probe is thus promising for investigation of the biological functions of LDs. |
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| Keywords: | Lipid droplets Twisted intramolecular charge transfer Long-term imaging Excellent photostability |
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