单细胞核酸编码扩增成像分析

单细胞成像可在单细胞水平观测目标物位置、 确定目标物含量, 在生命科学与临床医学研究领域应用广泛. 核酸编码扩增技术利用特定分子反应将待测目标识别转化为核酸条码的扩增, 具有探针种类多、 易编程、 反应条件温和及信号放大效率高等特点, 在单细胞低丰度、 高灵敏、 多目标物成像中优势显著, 为理解细胞状态、 探索生命过程提供了新思路. 本文综合评述了核酸编码扩增在单细胞荧光成像领域的研究进展, 以目标物的编码方式为分类依据, 系统阐述了固定细胞原位成像和活细胞成像中不同目标物编码与扩增成像方式的区别, 并对活细胞成像中多重检测面临的问题以及未来发展前景进行了展望.

Nucleic Acids-encoded Amplification for Single-cell Imaging

Single-cell imaging is of great significance in the field of life science and clinical medicine for its application on visualizing and quantifying of targets in single cells. Nucleic acids-encoded amplification converts targets to nucleic acids barcodes through specific molecular reactions， which is easy to achieve signal amplification. Due to its variety of probes， ease of programming， mild reaction conditions and high amplification efficiency， nucleic acids- encoded amplification emerges outstanding performance on sensitive and multiplexed imaging of various targets with low abundance in single cells， emerging as a new strategy for understanding cell state and exploring life process. This paper reviews the research progress of nucleic acids-encoded amplification in the field of single-cell fluorescence imaging on the basis of the encoding methods， which systematically clarifies the characteristics of encoding and amplification methods for living cell imaging and in situ cell imaging. Finally， the challenges of multiplex detection in living cells and future perspectives of nucleic acids-encoded amplification are summarized and discussed.