The Use of Electrospray Mass Spectrometry (ES/MS) for the Differential Detection of Some Steroids as Calix-[n]-arene Sulphonate Complexes |
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Authors: | Eric Da Silva Charléne Valmalle Michel Becchi Claude-Yves Cuilleron Anthony W. Coleman |
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Affiliation: | (1) IBCP, CNRS-UMR 5086, 7 passage du Vercors, 69367 Lyon, France, cedex;(2) Pathologie Hormonale Moléculaire, Institut National de la Santé et de la Recherche Médicale Unité INSERM U 329, Hôpital Debrousse, Lyon, France |
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Abstract: | Non-covalent inclusion complexes formed between three p-sulphonato-calix-[n]-arenes (1 n = 4, 2 n = 6 and 3 n = 8) and three steroids (progesterone, testosterone and oestradiol) have been studied by electrospray mass spectrometry (ES/MS). Mass spectrometric titration experiments have demonstrated differences with regard to selectivity of each p-sulphonato-calix-[n]-arene against the steroids. p-sulphonato-calix-[8]-arene interacts more strongly with oestradiol and p-sulphonato-calix-[6]-arene with progesterone. Studies in which different orifice voltages were applied show that all oestradiol complexes are reduced in signal intensity at 50 V as compared to 20 V, whereas the intensities observed for the testosterone and progesterone complexes do not vary with voltage. Competition experiments confirm the selectivity of the complexation. |
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Keywords: | steroids water-soluble calixarenes electrospray mass spectrometry complexation |
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