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PELDOR Spectroscopy Distance Fingerprinting of the Octameric Outer‐Membrane Protein Wza from Escherichia coli. |
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| Authors: | Gregor Hagelueken Dr. W. John Ingledew Dr. Hexian Huang Biljana Petrovic‐Stojanovska Chris Whitfield Prof. Hassane ElMkami Dr. Olav Schiemann Dr. James H. Naismith Prof. |
| Affiliation: | 1. Centre for Biomolecular Sciences, The University of St. Andrews, Fife KY16 9RH (UK);2. Department of Molecular and Cellular Biology, University of Guelph, Ontario N1G 2W1 (Canada);3. School of Physics and Astronomy, University of St Andrews, Fife KY16 9SS (UK) |
| Abstract: | Distance fingerprinting : Pulsed electron–electron double resonance spectroscopy (PELDOR) is applied to the octameric membrane protein complex Wza of E. coli. The data yielded a detailed distance fingerprint of its periplasmic region that compares favorably to the crystal structure. These results provide the foundation to study conformation changes from interaction with partner proteins.  |
| Keywords: | DEER spectroscopy EPR spectroscopy membrane proteins PELDOR spectroscopy spin labeling |