Simultaneous determination of anti‐diabetes/anti‐obesity drugs by LC/PDA,and targeted analysis of sibutramine analog in dietary supplements by LC/MS/MS |
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| Authors: | So Hyun Kim Jeongmi Lee Taehyung Yoon Jangduck Choi Dongmi Choi Deukjoon Kim Sung Won Kwon |
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| Affiliation: | 1. College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151742, Korea;2. So Hyun Kim and Jeongmi Lee contributed equally to this work.;3. Department of Pharmacology, The University of Texas Southwestern Medical Center at Dallas, Texas 75390, USA;4. Korea Food and Drug Administration, 194 Tongilro, Eunpyeong‐gu, Seoul 122704, Korea |
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| Abstract: | The safety of dietary supplements is questionable as there have been occasional reports of products contaminated with illegal adulterants. The present study was carried out to develop trustworthy methodologies to screen for six anti‐diabetic drugs (phenformin, rosiglitazone, glipizide, glimepiride, glybenclamide and gliclazide) and six anti‐obesity drugs (ephedrine, fenfluramine, T3, T4, fluoxetine and sibutramine) in dietary supplements. A simultaneous determination method of the 12 drugs by liquid chromatography coupled with a photodiode array (LC/PDA) was established and was validated for linearity (r2 > 0.99), precision (RSD <13.3%), recoveries (88.8–115.9%) and reproducibility. Sibutramine and its analogs, N‐desmethylsibutramine, were subject to further investigation by LC/MS/MS because they were one of the major illegal adulterants. Our proposed method to monitor illegal drug adulterations in dietary supplements using LC/PDA is a simple and reliable, and therefore applicable to routine drug‐adulteration screening. Copyright © 2009 John Wiley & Sons, Ltd. |
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| Keywords: | liquid chromatography photo diode array mass spectrometry adulterant |
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