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Efficient Replication Bypass of Size‐Expanded DNA Base Pairs in Bacterial Cells |
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| Authors: | James C. Delaney Dr. Jianmin Gao Dr. Haibo Liu Dr. Nidhi Shrivastav John M. Essigmann Prof. Dr. Eric T. Kool Prof. Dr. |
| Affiliation: | 1. Departments of Chemistry and Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139 (USA);2. Department of Chemistry, Stanford University, Stanford, CA 94305 (USA) |
| Abstract: | Supersize me! Size‐expanded DNA bases (xDNA) are able to encode natural DNA sequences in replication. In vitro experiments with a DNA polymerase show nucleotide incorporation opposite the xDNA bases with correct pairing. In vivo experiments using E. coli show that two xDNA bases (xA and xC, see picture) encode the correct replication partners.  |
| Keywords: | DNA replication nucleobases polymerase steric hindrance |