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Engineering A Uranyl‐Specific Binding Protein from NikR |
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| Authors: | Seraphine V Wegner Hande Boyaci Hao Chen Dr Mark P Jensen Dr Chuan He Prof |
| Institution: | 1. Department of Chemistry, The University of Chicago, 929 East 57th Street, Chicago, IL 60637 (USA), Fax: (+1)?773‐702‐0805;2. Chemical Sciences and Engineering Division, Argonne National Laboratory, 9700 S. Cass Avenue, Argonne, IL 60439 (USA) |
| Abstract: | A new pick‐up line : The first uranyl‐selective DNA‐binding protein is designed using the E. coli nickel(II)‐responsive protein NikR as the template. The resulting NikR′ protein binds uranyl (see picture) with a dissociation constant Kd=53 nM and selectively binds to DNA in the presence of uranyl.  |
| Keywords: | bioinorganic chemistry metalloproteins nickel protein engineering uranium |