Determination of tectoridin in rat plasma by high‐performance liquid chromatography and its application to pharmacokinetic studies |
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| Authors: | Taiming Liu Zhuo Chen Yuyi Guo Xuehua Jiang Guo Ma |
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| Institution: | 1. Key Laboratory of Drug Targeting, West China School of Pharmacy, Sichuan University, No. 17, Section 3, Southern Renmin Road, Chengdu 610041, People's Republic of China;2. School of Pharmacy, Fudan University, Shanghai, 200032, People's Republic of China |
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| Abstract: | A simple, specific and sensitive HPLC method with UV detection was developed and validated for the determination of tectoridin in rat plasma for the first time. Chromatographic separation was performed on a WelchromTM C18 column (150 × 4.6 mm, i.d., 5 µm) at a flow rate of 1.0 mL min?1, using a mixture of methanol–2% HAc aqueous solution (31:69, v/v) as the mobile phase with UV detection at 266 nm. The calibration curves for tectoridin were linear over the concentration range of 1.10–274.40 µg mL?1 in rat plasma. The intra‐ and inter‐day accuracies (RE) were within ?3.23% and 4.11%. The intra‐ and inter‐day precisions (RSD) were not more than 2.74 and 4.72%, respectively. The present method was successfully applied to the pharmacokinetic studies of tectoridin in rats after intravenous administration of three different doses. Copyright © 2009 John Wiley & Sons, Ltd. |
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| Keywords: | tectoridin pharmacokinetics high‐performance liquid chromatography plasma |
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