RP‐HPLC determination of phenylalkanoids and monoterpenoids in Rhodiola rosea and identification by LC‐ESI‐TOF |
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Authors: | Bharathi Avula Yan‐Hong Wang Zulfiqar Ali Troy J. Smillie Vicky Filion Alain Cuerrier John T. Arnason Ikhlas A. Khan |
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Affiliation: | 1. National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, MS 38677, USA;2. Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5;3. Plant Biology Research Institute, Université de Montréal, Montréal, Québec, Canada H1X 2B2;4. Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, MS 38677, USA |
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Abstract: | An HPLC method permitting the simultaneous determination of fourteen analytes (phenylalkanoids and monoterpenoids) from the roots of Rhodiola rosea was developed. A separation was achieved within 35 min using C18 column material and a water–acetonitrile mobile phase, both containing a 0.05% phosphoric acid gradient system and a temperature of 53°C. The method was validated for linearity, repeatability, limits of detection and limits of quantification. The limits of detection and limits of quantification of 14 phenylalkanoids and monoterpenoids were found to be 0.20–1.0 and 0.5–3.5 µg/mL, respectively. The wavelengths used for quantification of phenylalkanoids and monoterpenoids with a diode array detector were 205, 220 and 251 nm. The method was used to analyze the roots of two species of Rhodiola and commercial extracts of R. rosea and provides preliminary evidence of phytochemical differences between North American and Eurasian populations of R. rosea. LC–mass spectrometry coupled with electrospray ionization (ESI) interface method is described for the identification of phenylalkanoids and monoterpenoids in various Rhodiola samples. This method involved the use of the [M + H]+, [M + NH4]+ and [M + Na]+ ions in the positive ion mode with extractive ion monitoring (EIM). Copyright © 2009 John Wiley & Sons, Ltd. |
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Keywords: | Rhodiola species LC‐UV LC‐MS monoterpenoids phenylalkanoids |
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