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A Xenon‐129 Biosensor for Monitoring MHC–Peptide Interactions |
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| Authors: | Andreas Schlundt Wolfgang Kilian Dr. Michael Beyermann Dr. Jana Sticht Dr. Sebastian Günther Sabine Höpner Kirsten Falk Dr. Olaf Roetzschke Dr. Lorenz Mitschang Dr. Christian Freund Dr. |
| Affiliation: | 1. Protein Engineering Group, Leibniz‐Institut für Molekulare Pharmakologie und FU Berlin, Robert‐Roessle‐Strasse 10, 13125 Berlin (Germany), Fax: (+49)?30‐94793‐189;2. Physikalisch‐Technische Bundesanstalt, Berlin (Germany);3. Peptide Chemistry Group, Leibniz‐Institut für Molekulare Pharmakologie und FU Berlin;4. Max‐Delbrück‐Centrum für Molekulare Medizin, Berlin (Germany);5. The Singapore Immunology Network, Singapore 138648 (Singapore) |
| Abstract: | Caged in : The formation of a complex between a peptide ligand and a major histocompatibility complex (MHC) class II protein is detected by a 129Xe biosensor. Cryptophane molecules that trap Xe atoms are modified with a hemagglutinin (HA) peptide, which binds to the MHC protein. The interaction can be monitored by an NMR chemical shift change of cage–HA bound 129Xe.  |
| Keywords: | antigens biosensors NMR spectroscopy peptides xenon |