A rapid and simple HPLC method for the determination of curcumin in rat plasma: assay development,validation and application to a pharmacokinetic study of curcumin liposome |
| |
Authors: | Ji Li Yunyun Jiang Jun Wen Guorong Fan Yutian Wu Chuan Zhang |
| |
Affiliation: | 1. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, People's Republic of China;2. Shanghai Key Laboratory for Pharmaceutical Metabolite Research, No. 325 Guohe Road, Shanghai 200433, People's Republic of China;3. Shanghai Research Centre for Drug (Chinese Materia Medica) Metabolism, No. 325 Guohe Road, Shanghai 200433, People's Republic of China;4. Ji Li and Yunyun Jiang contributed equally to this work. |
| |
Abstract: | This paper describes a sensitive, specific and rapid high‐performance liquid chromatography (HPLC) method for the determination of curcumin in rat plasma. After a simple step of protein precipitation in 96‐well format using acetonitrile containing the internal standard (IS), emodin, plasma samples were analyzed by reverse‐phase HPLC. Curcumin and the IS emodin were separated on a Diamonsil C18 analytical column (4.6 × 100 mm, 5 µm) using acetonitrile–5% acetic acid (75:25, v/v) as mobile phase at a flow rate of 1.0 mL/min. The method was sensitive with a lower limit of quantitation of 1 ng/mL, with good linearity (r2 ≥ 0.999) over the linear range 1–500 ng/mL. All the validation data, such as accuracy and precision, were within the required limits. A run time of 3.0 min for each sample made high‐throughput bioanalysis possible. The assay method was successfully applied to the study of the pharmacokinetics of curcumin liposome in rats. Copyright © 2009 John Wiley & Sons, Ltd. |
| |
Keywords: | curcumin HPLC 96‐well protein precipitation pharmacokinetics |
|
|