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Semisynthesis of a Homogeneous Glycoprotein Enzyme: Ribonuclease C: Part 2 |
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| Authors: | Christian Piontek Dr Daniel Varón Silva Dr Christian Heinlein Claudia Pöhner Stefano Mezzato Dr Petra Ring Andreas Martin Dr Franz Xaver Schmid Prof Carlo Unverzagt Prof |
| Institution: | Bioorganische Chemie, Geb?ude NW1, Universit?t Bayreuth, 95440 Bayreuth (Germany), Fax: (+49)?921‐555‐365 |
| Abstract: | Active RNase glycoprotein from three pieces : The glycoprotein enzyme ribonuclease C, which contains a complex saccharide N‐glycan, was synthesized by sequential native chemical ligation. An optimized ligation and isolation protocol allowed the efficient assembly and refolding of the 124 amino acid enzyme.  |
| Keywords: | glycoproteins glycosylation native chemical ligation protecting groups ribonucleases |