Ion chromatography tandem mass spectrometry for simultaneous confirmation and determination of indandione rodenticides in serum |
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| Authors: | Xiao‐hong Chen Mei‐qiang Cai Xiao‐kun OuYang Mi‐cong Jin |
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| Affiliation: | 1. Ningbo Key Laboratory of Poison Research and Control, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China;2. College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310035, China;3. School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316004, China |
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| Abstract: | This paper describes a simple method for the simultaneous determination and confirmation of the indandione rodenticides in serum. After samples were extracted with 10% (v/v) methanol in acetonitrile and cleaned by solid‐phase extraction, chromatographic separation was performed on an IonPac® AS11 analytical column (250 × 4.0 mm) using gradient KOH eluent with 10% (v/v) methanol as organic modifier. Confirmation was depended on the extensive fragmentation of the indandione molecule under MS/MS conditions which provides sufficient structural information. Quantification was performed by negative electrospray ionization in multiple reaction monitoring mode. All the method parameters were validated. It was confirmed that this method could be used in clinical diagnosis and forensic toxicology. Copyright © 2009 John Wiley & Sons, Ltd. |
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| Keywords: | ion chromatography– tandem mass spectrometry indandione rodenticide serum multiple reaction monitoring |
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