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Luminescent Terbium Protein Labels for Time‐Resolved Microscopy and Screening |
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| Authors: | Harsha?E Rajapakse D?Rajasekhar Reddy Dr Shabnam Mohandessi Nathaniel?G Butlin Dr Lawrence?W Miller Prof?Dr |
| Institution: | 1. Department of Chemistry, University of Illinois at Chicago, 845 W. Taylor St., Chicago, IL 60607 (USA), Fax: (+1)?312‐996‐0431;2. Lumiphore, Inc. 4677 Meade St., Suite 216, Richmond, CA 94804 (USA) |
| Abstract: | Brilliance of terbium : Heterodimeric conjugates of trimethoprim covalently linked to sensitized terbium chelates bind to Escherichia coli dihydrofolate reductase fusion proteins with nanomolar affinity (see picture). Terbium luminescence enables sensitive and time‐resolved detection of labeled proteins in vitro and on the surface of living mammalian cells.  |
| Keywords: | FRET lanthanides protein labeling time‐resolved microscopy trimethoprim |