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Fluorescence Logic‐Signal‐Based Multiplex Detection of Nucleases with the Assembly of a Cationic Conjugated Polymer and Branched DNA
Authors:Xuli Feng  Xinrui Duan  Libing Liu Dr.  Fude Feng  Shu Wang Prof.  Yuliang Li Prof.  Daoben Zhu Prof.
Affiliation:Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 (P.R. China), Fax: (+86)?10‐6263‐6680
Abstract:An energy‐transfer cascade is generated from a cationic conjugated polymer (PFP) and negatively charged, Y‐shaped DNA labeled with three dyes at its termini (fluorescein (Fl), Tex Red, and Cy5). Multistep fluorescence resonance energy transfer regulates the fluorescence intensities of PFP and the dyes. Different types of logic gates can be operated by observing the emission wavelengths of different dyes with multiplex nucleases as inputs.
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Keywords:biosensors  conjugated polymers  DNA cleavage  FRET  nucleases
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